IHC & in situ Hybridization
  1. Triton® X-100
    Triton® X-100
    Catalog Number 04605

    A nonionic surfactant useful in solubilizing protein aggregates. May also be used as a parting agent to facilitate removal of gels from tube walls.

    Octylphenol-polyethyleneglycol ether; Octylphenoxypolyethoxyethanol

  2. Triton® X-100, 10% aqueous solution

    Under nitrogen in sealed ampoules.

  3. Hanker-Yates reagent
    Hanker-Yates reagent
    Catalog Number 08661

    A specific, sensitive and non-carcinogenic chromogen for immunoperoxidase techniques. Contains p-phenylenediamine and pyrocatechol. Gives a blue reaction product.

  4.  20X 10mM Citrate Buffer Kit, pH 6.0 (for antigen retrieval FFPE and resin)

    Formalin fixation forms protein cross-links that can mask the antigenic sites in tissue specimens, thereby creating weak or false negative staining for immunohistochemical detection of certain proteins. There are currently several antigen retrieval methods commonly used and the protocols below only intended as a general guide. Polysciences, Inc. provides AR buffer kits, that include rinses and pH adjustment reagents for the 20X concentration kit. These kits are recommended for formalin fixed paraffin embedded tissues.

  5. 1X 10mM Citrate Buffer Kit, pH 6.0 (for antigen retrieval FFPE and resin)
    Inquire for availability.

    Formalin fixation forms protein cross-links that can mask the antigenic sites in tissue specimens, thereby creating weak or false negative staining for immunohistochemical detection of certain proteins. There are currently several antigen retrieval methods commonly used and the protocols below only intended as a general guide. Polysciences, Inc. provides AR buffer kits, that include rinses and pH adjustment reagents for the 20X concentration kit. These kits are recommended for formalin fixed paraffin embedded tissues.

  6. L.A.B. Solution (Liberate Antibody Binding Solution)

    L.A.B. Solution liberates your antibody binding sites for improved immunohistochemical (IHC) staining.

  7. Melanin Bleach Kit
    Melanin Bleach Kit
    Catalog Number 24883

    Remove melanin pigment in cells of malignant melanomas and reveal cellular detail!

  8. Delicate Melanin Bleach Kit for Special Stains and IHC

    Delicate formula for removing melanin pigment in cells of malignant melanomas created specially for Special Stains and IHC.

  9. Poly(ethylene glycol) distearate 6,000

    A removable embedding medium for thin and thick sections. In thicker sections the exterior surface of mitochondria can be observed whereas in resin-embedded thin sections the mitochondria are most frequently observed in a cross section. DGD is used to prepare tissue for immunofluorescent localization of cytoskeletal components. Also used for tissue preparation for in-situ hybridization with nucleic acid probes.

    MW = Molecular weight of PEG.

  10. Sodium Dodecyl Sulfate (SDS) Anionic Surfactant

    Anionic surfactant, which will decrease polymer bead hydrophobicity and can additionally participate in charge stabilization of the suspension. SDS is a more rigorous surfactant than is commonly used in uncoated polymer bead preparations.

  11. Anti-Biotin in rabbit, IgG fraction, lyophilized

    Anti-Biotin in rabbit, IgG fraction, lyophilized.

  12. Anti-Albumin (human serum) in rabbit, IgG fraction

    This polyclonal antibody reacts with all forms of human serum albumin and will cross- react with most mammalian albumins. This antibody efficiently precipitates protein and can be used in a variety of detection and capture techniques.

    Suitable for: ELISA, Western Blotting, Immunoprercipitation

  13. Anti-Transferrin (human) in rabbit, IgG fraction

    This Antibody reacts with transferrin from human serum. Strong cross-reactivity will occur with mammalian forms of this protein.

  14. Anti-Ovalbumin (egg white) in rabbit, IgG fraction

    Antibody reacts with ovalbumin from hen egg white showing a 45 kDa band under non-reducing and reducing conditions of SDS-PAGE.

    Suitable for: ELISA, Western Blotting, Immunoprecipitation

  15. Anti-Esterase (porcine liver) in rabbit, IgG fraction

    This Antibody reacts with esterase from porcine liver. Cross-reactivity is expected against most mammalian forms of this enzyme.

    Suitable for: ELISA, Western Blotting

  16. Anti-Goat IgG (H&L) in rabbit

    (Secondary antibodies and conjugates, affinity purified) We recommend that each investigator determine the actual optimum dilution for a given antibody or antibody conjuagte for each specific assay used. Enzyme conjugates of affinity purified antibodies are supplied with lot-specific recommended dilutions.

    liquid

  17. Anti-Human IgG (H&L) in sheep

    (Secondary antibodies and conjugates, affinity purified) We recommend that each investigator determine the actual optimum dilution for a given antibody or antibody conjugate for each specific assay used. Enzyme conjugates of affinity purified antibodies are supplied with lot-specific recommended dilutions.

    liquid

  18. Anti-Human IgG (H&L) in goat, rhodamine conjugate

    (Secondary antibodies and conjugates, affinity purified) We recommend that each investigator determine the actual optimum dilution for a given antibody or antibody conjugate for each specific assay used. Enzyme conjugates of affinity purified antibodies are supplied with lot-specific recommended dilutions.

    rhodamine conjugate, lyophilized

  19. Anti-Human IgG (H&L) in sheep, rhodamine conjugate

    (Secondary antibodies and conjugates, affinity purified) We recommend that each investigator determine the actual optimum dilution for a given antibody or antibody conjugate for each specific assay used. Enzyme conjugates of affinity purified antibodies are supplied with lot-specific recommended dilutions.

    rhodamine conjugate, lyophilized

  20. Anti-Human IgG (H&L) in goat, Texas red conjugate

    (Secondary antibodies and conjugates, affinity purified) We recommend that each investigator determine the actual optimum dilution for a given antibody or antibody conjugate for each specific assay used. Enzyme conjugates of affinity purified antibodies are supplied with lot-specific recommended dilutions.

    Texas red conjugate, lyophilized

  21. Anti-Human IgG (H&L) in goat, biotin conjugate

    (Secondary antibodies and conjugates, affinity purified) We recommend that each investigator determine the actual optimum dilution for a given antibody or antibody conjugate for each specific assay used. Enzyme conjugates of affinity purified antibodies are supplied with lot-specific recommended dilutions.

    biotin conjugate, lyophilized

  22. Anti-Human IgG (H&L) in sheep, biotin conjugate

    (Secondary antibodies and conjugates, affinity purified) We recommend that each investigator determine the actual optimum dilution for a given antibody or antibody conjugate for each specific assay used. Enzyme conjugates of affinity purified antibodies are supplied with lot-specific recommended dilutions.

    biotin conjugate, lyophilized

  23. Anti-Human IgG (H&L) in sheep, alkaline phosphatase conjugate

    (Secondary antibodies and conjugates, affinity purified) We recommend that each investigator determine the actual optimum dilution for a given antibody or antibody conjugate for each specific assay used. Enzyme conjugates of affinity purified antibodies are supplied with lot-specific recommended dilutions.

    alkaline phosphatase conjugate, liquid

  24. Anti-Human IgG (H&L) in goat, fluoroscein conjugate

    (Secondary antibodies and conjugates, affinity purified) We recommend that each investigator determine the actual optimum dilution for a given antibody or antibody conjugate for each specific assay used. Enzyme conjugates of affinity purified antibodies are supplied with lot-specific recommended dilutions.

    (minimum cross-reactivity to Bv, Ch, GP, Gt, Ham, Hs, Ms, Rb, Rt & Sh serum proteins), fluorescein conjugate, lyophilized

  25. Anti-Human IgG (H&L) in goat, rhodamine conjugate

    (Secondary antibodies and conjugates, affinity purified) We recommend that each investigator determine the actual optimum dilution for a given antibody or antibody conjugate for each specific assay used. Enzyme conjugates of affinity purified antibodies are supplied with lot-specific recommended dilutions.

    (minimum cross-reactivity to Bv, Ch, GP, Gt, Ham, Hs, Ms, Rb, Rt & Sh serum proteins), rhodamine conjugate, lyophilized

  26. Bovine serum albumin (fraction V, protease-free), lyophilized
    Inquire for availability.

    Blocking reagent, lyophilized.

  27. DAB-4HCl, Immunochemical Grade, 10mg Lyophilized per vial

    Chromogen of choice for immunoperoxidase techniques.

  28. Sodium dodecyl sulfate
    Sodium dodecyl sulfate
    Catalog Number 03945
    • Assay as C12: 99+% min.
    • Heavy metals: 10ppm max.
  29. Octyl D-glucopyranoside
    Octyl D-glucopyranoside
    Catalog Number 14528
    Octyl a-D-glucopyranoside is a detergent that can be used as a substrate to measure rates of alcohol dehyrogenase and to remove glycol ethers from biological samples.
  30. Dodecyl maltoside
    Dodecyl maltoside
    Catalog Number 14529
    Dodecyl maltoside is a non-ionic detergent. Due to it's low critical micelle concentration, it is considered gentle but powerful. It can be used to solubilize or crystalize membrane proteins while preservering their structure
  31. Sucrose monocaprate
    Sucrose monocaprate
    Catalog Number 14530
    Sucrose monocaprate is a non-ionic detergent.
  32. Digitonin
    Digitonin
    Catalog Number 14533
    Digitonin is a non ionic surfactant used to solubilize membrane bound proteins and to permeabilize cell membranes.
  33. Tocofersolan
    Tocofersolan
    Catalog Number 14542
    Tocofersolan is a nonionic surfactant and a derivative of vitamin E. It can be used with lipids like DOTMA to formulate liposomes for a variety of antitumor applications.
  34. Tween 60
    Tween 60
    Catalog Number 14560
    Tween 60 is a nonionic detergent used for forming oil in water emulsions.
  35. Tween 80
    Tween 80
    Catalog Number 14561
    Tween 80 is a nonionic detergent and emulsifier used in pharmaceuticals.
  36. Tween 85
    Tween 85
    Catalog Number 14562
    Tween 85 is a nonionic detergent used for forming oil in water emulsions.
  37. Tween 40
    Tween 40
    Catalog Number 14563
    Tween 40 is a non ionic detergent.
  38. Tween 80 (Suitable for cell culture)
    Tween 80 is a nonionic detergent and emulsifier used in cell culture.
  39. Anti-Human Neuron specific enolase (NSE) in rabbit, serum, lyophilized

    Both antisera (Cat. 16625 and Cat. #17437) are very specific and are the reagents of choice for the visualization of neurons and peptide secreting neuroendocrine cells by immunocytochemistry.

  40. Anti-Rat Neuron specific enolase (NSE) in rabbit, serum, lyophilized

    Both antisera Cat. #16625 and Cat. #17437 are very specific, and are the reagents of choice for the visualization of neurons and peptide secreting neuroendocrine cells by immunocytochemistry.

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