The identification of Mycobacteria with rhodamine-auramine is due to the affinity of the mycolic acid in the cell walls for the fluorochromes.
Dyes will bind to the Mycobacteria, which appear bright yellow or orange against a greenish background. The potassium permanganate helps prevent non-specific fluorescence. All acid-fast organisms will be stained by rhodamine-auramine, including the sporozoan parasites.
Slides stained with rhodamine-auramine may be restained with Ziehl-Neelsen or Kinyoun stain directly, as long as the oil has been removed. This provides a convenient method of confirming and differentiating morphology of positive slides with the traditional stains.
Kit includes: Rhodamine - Auramine, Fluorescent Decolorizer, Potassium - Permanganate (8 oz. bottle of each)