Neuronal Tracing

Fluorescent Neuronal Tracers

Fast Blue (FB), Diamidino Yellow (DY), True Blue (TB) and Granular Blue (GB) are fluorescent dyes most commonly used as retrograde neuronal tracers.

FB is the tracer of choice for retrograde motoneuronal labeling in long-term experiments. FB can be used alone or with other types of fluorescent tracer dyes to map neuronal pathways.

When combined with FB, TB, or GB, DY is effective in double-label experiments aimed at demonstrating the existence of divergent axon collaterals.

Catalog # Name Excitation (nm) Emission (nm)
17740 Fast Blue (FB) 365 420
26274 Diamidino Yellow (DY) Diaceturate 365 > 500
26272 True Blue (TB) Diaceturate Salt 365 405
26273 Granular Blue (GB) Dihydrochloride 365 410
  1. NeuroVue® Red  - Filter Square For Neuronal Tract Tracing

    NeuroVue® Dye Filter is useful for neuronal tract tracing studies of up to 3-4 weeks. Spectrally compatible with eGFP, YFP in many systems, and NeuroVue® Maroon & Jade. Spectral unmixing required for use with NeuroVue® Orange. 1 cm² nylon filter coated with the lipophilic red emitting dye, NeuroVue® Red. Typical dye loading: 11-14nmoles/mm².

  2. NeuroVue® Orange - Filter Square For Neuronal Tract Tracing

    NeuroVue® Dye Filter is useful for neuronal tract tracing studies of up to 3-4 weeks. Spectrally compatible with eGFP and YFP in many systems, and NeuroVue® Maroon, & Jade. Spectral unmixing required for use with NeuroVue® Red. 1cm² filter coated with the lipophilic reddish orange emitting dye, NeuroVue® Orange (ex max=550nm; em max=570nm).

  3. NeuroVue® Jade - Filter Square For Neuronal Tract Tracing

    Useful for neuronal tract tracing studies of up to five days. Spectrally compatible with NeuroVue® Maroon, Orange & Red. 1cm² nylon filter coated with the lipophilic green emitting dye, NeuroVue® Jade.

    Typical dye loading: 22-30 nmoles/mm,sup>2.

  4. NeuroVue® Burgundy - Filter Square For Neuronal Tract Tracing

    Useful for tract tracing studies of up to 7 days. Spectrally compatible with most fluorescent genetic tags and NeuroVue® Red, Orange, Jade. Spectral unmixing required for use with NeuroVue® Maroon. 1cm² filter coated with lipophilic far red emitting dye, NeuroVue® Burgundy (ex max=683nm; em max=707nm).

  5. NeuroVue® Red Plus - Filter Square For Neuronal Tract Tracing

    Useful for tract tracing studies of up to 3-4 weeks. Spectrally compatible with eGFP, YFP in many systems, and NeuroVue® Maroon and Jade. Spectral unmixing required for use with NeuroVue® Orange. NeuroVue® Red Plus provides faster and more efficient neuron labeling with higher dye loading than standard NeuroVue® Red filter square.

    1 cm2 nylon filter coated with the lipophilic red emitting dye, NeuroVue Red.

    Typical dye loading: 18-21nmoles/mm2.

  6. NeuroVue® Red Solid - For Neuronal Tract Tracing

    NeuroVue® Red Solid can be formulated as desired for applications in which the filter format is not suitable. NeuroVue® Red Solid is useful for neuronal tract tracing studies of up to 3-4 weeks. Spectrally compatible with eGFP, YFP in many systems, and NeuroVue® Maroon & Jade. Spectral unmixing required for use with NeuroVue® Orange.

  7. Dextran, FITC
    Dextran, FITC
    Catalog Number 15759

    Fluorescein isothiocyanate (FITC) labeled Dextrans are valuable materials for studying permeability and microcirculation in vivo. These are used to trace neuronal projections and active transport in live and unfixed tissue and as neuronal tracers in a variety of species.

  8. NeuroVue® Jade Solid
    NeuroVue® Jade Solid
    Catalog Number 25687

    For tracing neuronal connections. Dye can be melted or dissolved in solvent then be used for application into small injection sites.

    1 mg of solid dye can be formulated as desired for application.(ex max= 478nm and em max=508nm)

  9. True Blue (TB) Diaceturate Salt
    Inquire for availability.
    True Blue (TB) is a fluorescent retrograde tracer used in experimental studies of the peripheral nervous system to determine the number and origin of neurons projecting to a specific area.
  10. Granular Blue (GB) Dihydrochloride
    Inquire for availability.

    Granular Blue (GB) is a fluorescent retrograde tracer used in experimental studies of the peripheral nervous system to determine the number and origin of neurons projecting to a specific area.

  11. Fast Blue
    Fast Blue (FB)
    Catalog Number 17740

    Fast blue fluorescent tracer used in studies of peripheral nervous system to determine the number & origin of neurons projecting to specific area 

  12. NeuroVue® Maroon - Filter Square For Neuronal Tract Tracing

    NeuroVue® Dye Filter is useful for neuronal tract tracing studies of up to 3-4 weeks. Spectrally compatible with most fluorescent genetic tags, and NeuroVue® Red, Orange & Emerald. 1cm2 filter coated with the lipophilic far red emitting dye, NeuroVue® Maroon (ex max=647nm; em max=667nm).

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