Transporter 5™ is a premium ready-to-use transfection reagent prepared from our popular linear polyethylenimine MAX (PEI MAX 1 mg/mL). Transporter 5™ effectively transfects mammalian and insect cells, especially HEK-293, CHO, and Sf9.
We understand every transfection is a major investment, so we designed Transporter 5™ to be a reliable reagent in any process.
The quality of Transporter 5™ begins with our production process. Our special production method for PEI creates pure polymer with no batch-to-batch molecular weight variation. Transporter 5™ combines our consistent PEI with the highest quality USP/NF reagents in sterile, single-use equipment. While standard methods use 0.2µm sterile-filters, we use 0.1µm to completely eliminate mycoplasma risk.
Our Quality Control ensures Transporter 5™ is the most reliable reagent available. We developed our own scientifically-sound, quantitative performance assay for Transporter 5™. Most commercial performance assays are based on qualitative assays like SEAP and GFP. These assays cannot ensure the high titers most scientists require. The few reagents that are tested quantitatively typically use undisclosed HDAC inhibitors to improve results. Transporter 5™ is required to produce high titers without any supplementation. Even after initial quality control, we continuously monitor performance to ensure each batch is as good as when it was created.
Rajendra, Y., Kiseljak, D., Baldi, L., Wurm, F. M. and Hacker, D. L. (2015), Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells. Biotechnol Progress, 31: 541–549. doi: 10.1002/btpr.2064
Longo, P. A., Kavran, J. M., Kim, M. & Leahy, D. J. Transient Mammalian Cell Transfection wtih Polyethylenimine (PEI). Methods Enzymology 529, 227–240 (2013). doi:10.1016/B978-0-12-418687-3.00018-5.
Wulhfard, S., Baldi, L., Hacker, D. L. & Wurm, F. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of Biotechnology 148, 128–132 (2010). doi:10.1016/j.jbiotec.2010.05.003
Backliwal, G., Hildinger, M., Hasija, V. and Wurm, F. M. (2008), High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI. Biotechnol. Bioeng., 99: 721–727. doi: 10.1002/bit.21596
Backliwal, G. et al. Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions. Nucleic Acids Research 36, e96–e96 (2008). doi:10.1093/nar/gkn423
Tom, R., Bisson, L. & Durocher, Y. Transfection of HEK293-EBNA1 cells in suspension with linear PEI for production of recombinant proteins. Cold Spring Harbor Protocols 3, 1–5 (2008). doi:10.1101/pdb.prot4977
Choosakoonkriang, S., Lobo, B. A., Koe, G. S., Koe, J. G. and Middaugh, C. R. (2003), Biophysical characterization of PEI/DNA complexes. J. Pharm. Sci., 92: 1710–1722. doi: 10.1002/jps.10437
Expired Patent: US6013240; Nucleic Acid Containing Composition, Preparation, and Uses of the Same
- Harmless, use normal precautions
- Gloves & chemical googles
- Store at 4 degree C; do not freeze. Shipped at room temperature (RT).