Products

True Blue (TB) Diaceturate Salt

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Catalog No. Packaging Size Price Quantity
26272-1 1 mg
€91.00
26272-5 5 mg
€364.00

Description

True Blue (TB) is a fluorescent retrograde tracer used in experimental studies of the peripheral nervous system to determine the number and origin of neurons projecting to a specific area.

In rats, TB is effectively transported over long distances, from the spinal cord to the cerebral cortex, to produce blue fluorescent labeling of neuronal cell body cytoplasm, nucleolus, proximal dendrites and axons. When combined with Diamidino Yellow (DY), TB is effective in double labeling experiments aimed at demonstrating the existence of divergent axon collaterals.

Excitation: 365 nm
Emission: 405 nm
CAS#:
108321-12-6
Formula:

C20H16N4O2 · 2C4H7NO3


Molecular Weight:
578.58
Soluble In:
Watyer, Acetic acid
Appearance:
Powder

Reference(s):

Wouterlood, F. G. A survey of current neuroanatomical tracing techniques. Neural Tracing Methods: Tracing Neurons and Their Connection. Neuromethods 92,1–49 (2014). doi:10.1007/978-1-4939-1963-5_1

Bertelli, J. A. & Mira, J.-C. Nerve repair using freezing and fibrin glue: Immediate histologic improvement of axonal coaptation. Microsurgery 14, 135–140 (1993). doi:10.1002/micr.1920140210

Kuypers, H.G.J.M., Huisman, A.M. Fluorescent neuronal tracersAdv. Cellular. Neurobiol. 5, 307-340 (1984).

Skirboll, L. et al. A method for specific transmitter identification of retrogradely labeled neurons: Immunofluorescence combined with fluorescence tracing. Brain Research Reviews 8, 99–127 (1984). doi:10.1016/0165-0173(84)90001-8

Keizer, K., Kuypers, H. G. J. M., Huisman, A. M. & Dann, O. Diamidino yellow dihydrochloride (DY.2HCl); a new fluorescent retrograde neuronal tracer, which migrates only very slowly out of the cell. Experimental Brain Research 51, 179-191 (1983). doi:10.1007/BF00237193

Björklund, A. & Skagerberg, G. Simultaneous use of retrograde fluorescent tracers and fluorescence histochemistry for convenient and precise mapping of monoaminergic projections and collateral arrangements in the CNS. Journal of Neuroscience Methods 1, 261–277 (1979). doi:10.1016/0165-0270(79)90038-4


Storage:
Store at room temperature

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