Flow Cytometry Standards

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  1. Size Calibration Standards | Polysciences, Inc.

    Size Calibration Standards

    Starting at: €411.00

    Size Calibration Standards include five highly uniform unlabeled microbead populations of different diameters (~4-11µm), which can be used to construct calibration plots for electronic volume and light scatter instrumentation.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

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  2. Fluoresbrite® Calibration Grade Size Range Kit

    Fluoresbrite® Calibration Grade Size Range Kit

    Starting at: €745.00

    Our flow cytometry customers demand precisely manufactured microparticles as calibration tools. Our unique synthesis methods routinely result in particles with fluorescence CV’s less than 5% and often as low as 1%. This kit guarantees that our discriminating customer will have the finest particles with the lowest fluorescence CV currently available from our stock.

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  3. Ultra Rainbow Fluorescent Particles ~3.8µm | Polysciences, Inc.

    Ultra Rainbow Fluorescent Particles ~3.8µm

    Starting at: €167.00

    Ultra Rainbow Fluorescent Particles are internally labeled with a mixture of fluorophores which enables them to be excited at wavelengths from 365nm to 650nm.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter to approximate the size of human lymphocytes.

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  4. Ultra Rainbow Fluorescent Particles ~10.2µm | Polysciences, Inc.

    Ultra Rainbow Fluorescent Particles ~10.2µm

    Starting at: €167.00

    Ultra Rainbow Fluorescent Particles are internally labeled with a mixture of fluorophores which enables them to be excited at wavelengths from 365nm to 650nm.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter to approximate the size of human lymphocytes.

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  5. Time Delay Calibration Standard | Polysciences, Inc.

    Time Delay Calibration Standard

    Starting at: €167.00

    Our Time Delay Calibration Standard is intended for use in assessing the delay between blue and red lasers. It features ~6µm microspheres dyed with a fluorophore that is excited with 488nm or 635nm excitation, and exhibits red / far-red emission.

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  6. Full Spectrum™

    Full Spectrum™

    Starting at: €167.00

    Multi-color Reference Standards are highly uniform microspheres which fluoresce over a broad range of the spectrum to provide several reference positions in multi-detector analysis. This standard is used to properly align your flow cytometer excited from UV to near IR; this standard emits over the entire spectrum.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter to approximate the size of human lymphocytes.

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  7. Alexa Fluor® 488 Reference Standard

    Alexa Fluor® 488 Reference Standard

    Starting at: €167.00

    Single-color Fluorescence Reference Standards are labeled with specific fluorochromes to exhibit the same spectral characteristics as labeled cells. Their size approximates that of human lymphocytes (~7-9µm in diameter) and are available in a wide vareity of fluorescent dyes. Fluorescein is also available as a 2µm bead. These standards may be used to QC a specific path of the optical system (laser/filter/PMT), to optimize filter and mirror sets for fluorophores, and to establish a test-specific Target Channel Value for instrument set-up.

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  8. Alexa Fluor® 647 Reference Standard

    Alexa Fluor® 647 Reference Standard

    Starting at: €167.00

    Single-color Fluorescence Reference Standards are labeled with specific fluorochromes to exhibit the same spectral characteristics as labeled cells. Their size approximates that of human lymphocytes (~7-9µm in diameter) and are available in a wide vareity of fluorescent dyes. Fluorescein is also available as a 2µm bead. These standards may be used to QC a specific path of the optical system (laser/filter/PMT), to optimize filter and mirror sets for fluorophores, and to establish a test-specific Target Channel Value for instrument set-up.

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  9. Certified Blank™ (no label) Reference Standard

    Certified Blank™ (no label) Reference Standard

    Starting at: €167.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

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  10. Fluorescein Reference Standard

    Fluorescein Reference Standard

    Starting at: €167.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

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  11. Propidium Iodide Reference Standard

    Propidium Iodide Reference Standard

    Starting at: €167.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

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  12. Texas Red Reference Standard

    Texas Red Reference Standard

    Starting at: €167.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

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  13. Hoechst 33342 Reference Standard

    Hoechst 33342 Reference Standard

    Starting at: €167.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

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  14. Acridine Orange Reference Standard

    Acridine Orange Reference Standard

    Starting at: €167.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

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  15. Chlorophyll Reference Standard

    Chlorophyll Reference Standard

    Starting at: €167.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

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  16. R-Phycoerythrin Reference Standard

    R-Phycoerythrin Reference Standard

    Starting at: €167.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

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  17. Allophycocyanine Reference Standard

    Allophycocyanine Reference Standard

    Starting at: €167.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

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  18. T.M. Rhodamine Reference Standard

    T.M. Rhodamine Reference Standard

    Starting at: €167.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

    Learn More
  19. DAPI Reference Standard

    DAPI Reference Standard

    Starting at: €167.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

    Learn More
  20. PE-Cy5 Reference Standard

    PE-Cy5 Reference Standard

    Starting at: €167.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

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  21. PE-TR Reference Standard

    PE-TR Reference Standard

    Starting at: €167.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

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  22. APC-Cy7 Reference Standard

    APC-Cy7 Reference Standard

    Starting at: €167.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

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  23. Violet Laser Reference Standard

    Violet Laser Reference Standard

    Starting at: €167.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

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  24. Cy™ 5 Reference Standard

    Cy™ 5 Reference Standard

    Starting at: €167.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

    Supplied as 2 x 7ml.

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  25. PE-Cy™ 7 Reference Standard

    PE-Cy™ 7 Reference Standard

    Starting at: €159.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

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  26. Fluoresbrite® YG Microspheres, Calibration Grade 0.50μm

    Fluoresbrite® YG Microspheres, Calibration Grade 0.50μm

    Starting at: €162.00

    This special grade of Fluoresbrite® Yellow Green Microspheres has been evaluated both for particle diameter, as well as the uniformity of the fluorescent dye distribution. Instrument manufacturers have demanded these high quality particles for their flow cytometry standards.

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  27. Fluoresbrite® YG Microspheres, Calibration Grade 1.00μm

    Fluoresbrite® YG Microspheres, Calibration Grade 1.00μm

    Starting at: €185.00

    This special grade of Fluoresbrite® Yellow Green Microspheres has been evaluated both for particle diameter, as well as the uniformity of the fluorescent dye distribution. Instrument manufacturers have demanded these high quality particles for their flow cytometry standards.

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  28. Fluoresbrite® YG Microspheres, Calibration Grade 2.00μm

    Fluoresbrite® YG Microspheres, Calibration Grade 2.00μm

    Starting at: €220.00

    This special grade of Fluoresbrite® Yellow Green Microspheres has been evaluated both for particle diameter, as well as the uniformity of the fluorescent dye distribution. Instrument manufacturers have demanded these high quality particles for their flow cytometry standards.

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  29. Fluoresbrite® YG Microspheres, Calibration Grade  3.00μm

    Fluoresbrite® YG Microspheres, Calibration Grade 3.00μm

    Starting at: €220.00

    This special grade of Fluoresbrite® Yellow Green Microspheres has been evaluated both for particle diameter, as well as the uniformity of the fluorescent dye distribution. Instrument manufacturers have demanded these high quality particles for their flow cytometry standards.

    Learn More
  30. Fluoresbrite® YG Microspheres, Calibration Grade 6.00μm

    Fluoresbrite® YG Microspheres, Calibration Grade 6.00μm

    Starting at: €284.00

    This special grade of Fluoresbrite® Yellow Green Microspheres has been evaluated both for particle diameter, as well as the uniformity of the fluorescent dye distribution. Instrument manufacturers have demanded these high quality particles for their flow cytometry standards.

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  31. Quantum™ QC, 8 Peak Beads

    Quantum™ QC, 8 Peak Beads

    Starting at: €215.00

    Quantum™ QC is a multi-intensity, multi-fluorescent standard that is intended for use as an in-depth tool for daily cytometer QC, and is appropriate for use with all lasers and detectors.

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  32. Flow Check™ YG 6.0 Unlabeled

    Flow Check™ YG 6.0 Unlabeled

    Starting at: €194.00

    Flow cytometry is a highly accurate technology used for cell measurements. High quality particles, supplied from Polysciences, are used to calibrate these instruments.

    This product is a component of the Flow Check™ YG 6.0 Kit (Cat. #23512), which allows comparision of various levels of green fluorescence. The full kit contains 4 components, each of which is packaged with ~2 x 106 particles per ml: a full intensity bead, two intermediate intensity beads and a blank bead.

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  33. Flow Check™ YG 2.0 Unlabeled

    Flow Check™ YG 2.0 Unlabeled

    Starting at: €198.00

    Flow cytometry is a highly accurate technology used for cell measurements. High quality particles, supplied from Polysciences, are used to calibrate these instruments.

    This product is a component of the Flow Check™ YG 2.0 Kit (Cat. #23513), which allows the comparison of various levels of green fluorescence. The full kit contains 4 components, each of which is packaged with ~1 x 107 particles per ml: a full intensity bead, two intermediate intensity beads and a blank bead. The CV's of these particles offer excellent size and fluorescence CV control.

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  34. Flow Check™ YG 2.0 Low Intensity Level 1

    Flow Check™ YG 2.0 Low Intensity Level 1

    Starting at: €291.00

    Flow cytometry is a highly accurate technology used for cell measurements. High quality particles, supplied from Polysciences, are used to calibrate these instruments.

    This product is a component of the Flow Check™ YG 2.0 Kit (Cat. #23513), which allows the comparison of various levels of green fluorescence. The full kit contains 4 components, each of which is packaged with ~1 x 107 particles per ml: a full intensity bead, two intermediate intensity beads and a blank bead. The CV's of these particles offer excellent size and fluorescence CV control.

    Learn More
  35. Flow Check™ YG 2.0 Low Intensity Level 2

    Flow Check™ YG 2.0 Low Intensity Level 2

    Starting at: €284.00

    Flow cytometry is a highly accurate technology used for cell measurements. High quality particles, supplied from Polysciences, are used to calibrate these instruments.

    This product is a component of the Flow Check™ YG 2.0 Kit (Cat. #23513), which allows the comparison of various levels of green fluorescence. The full kit contains 4 components, each of which is packaged with ~1 x 107 particles per ml: a full intensity bead, two intermediate intensity beads and a blank bead. The CV's of these particles offer excellent size and fluorescence CV control.

    Learn More
  36. Flow Check™ YG 6.0 Low Intensity Level 1

    Flow Check™ YG 6.0 Low Intensity Level 1

    Starting at: €297.00

    Flow cytometry is a highly accurate technology used for cell measurements. High quality particles, supplied from Polysciences, are used to calibrate these instruments.

    This product is a component of the Flow Check™ YG 6.0 Kit (Cat. #23512), which allows comparision of various levels of green fluorescence. The full kit contains 4 components, each of which is packaged with ~2 x 106 particles per ml: a full intensity bead, two intermediate intensity beads and a blank bead.

    Learn More
  37. Flow Check™ YG 6.0  Low Intensity Level 2

    Flow Check™ YG 6.0 Low Intensity Level 2

    Starting at: €297.00

    Flow cytometry is a highly accurate technology used for cell measurements. High quality particles, supplied from Polysciences, are used to calibrate these instruments.

    This product is a component of the Flow Check™ YG 6.0 Kit (Cat. #23512), which allows comparision of various levels of green fluorescence. The full kit contains 4 components, each of which is packaged with ~2 x 106 particles per ml: a full intensity bead, two intermediate intensity beads and a blank bead.

    Learn More
  38. Flow Check™ YG Kit 6.0

    Flow Check™ YG Kit 6.0

    Starting at: €797.00

    The particles in this kit allow comparision of various levels of green fluorescence. Kit contains 4 components, each packaged with ~2 x 106 particles per ml: a full intensity bead, two intermediate intensity beads and a blank bead.

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  39. Flow Check™ YG Kit 2.0

    Flow Check™ YG Kit 2.0

    Starting at: €776.00

    The particles in this kit allow comparison of various levels of green fluorescence. Kit contains 4 components packaged with ~1 x 107 particles per ml: a full intensity bead, two intermediate intensity beads and a blank bead. The CV's of these particles offer excellent size and fluorescence CV control.

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  40. Flow Check™ Undyed Particles

    Flow Check™ Undyed Particles

    Starting at: €160.00

    Flow Check™ Undyed Particles are 6µm in size and are supplied with Flow Check® Intensity Sets and Maxi-Brite Color Compensation Set as a comparison against dyed particles and cells.

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  41. Flow Check™ Ruby Red Fluorescent Microspheres

    Flow Check™ Ruby Red Fluorescent Microspheres

    Starting at: €209.00

    Flow Check™Ruby Red Fluorescent Microspheres are ~6µm narrow distribution polystyrene particles that can be used for flow cytometry applications where fluorescence emission in the range of 600nm to 710nm is needed. Suitable for 488nm and 663nm excitation, detection in PE-Cy5 or APC channels. Supplied as a 5ml suspension (~5 x 106 beads/ml) in convenient dropper bottle.

    • Excitation maximum: 475nm
    • Emission maximum: 663nm
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  42. Pacific Blue™ Reference Standard

    Pacific Blue™ Reference Standard

    Starting at: €167.00

    Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

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  43. Simply Cellular® anti-Mouse Compensation Standard

    Simply Cellular® anti-Mouse Compensation Standard

    Starting at: €282.00

    The Simply Cellular® anti-Mouse Compensation Standard is a mixture of two Simply Cellular® anti-Mouse IgG particle populations capable of binding high and low levels of mouse monoclonal antibody used in your assay. Ideal for performing compensation in multicolor (2, 3, 4, or more) analysis.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

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  44. Simply Cellular® anti-Rat Compensation Standard

    Simply Cellular® anti-Rat Compensation Standard

    Starting at: €282.00

    The Simply Cellular® anti-Rat Compensation Standard is a mixture of two Simply Cellular® anti-Rat IgG particle populations capable of binding high and low levels of rat monoclonal antibody used in your assay. Ideal for performing compensation in multicolor (2, 3, 4, or more) analysis.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

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  45. Simply Cellular® anti-Human Compensation Standard

    Simply Cellular® anti-Human Compensation Standard

    Starting at: €268.00

    Used in conjunction with hardware or software to remove spectral overlap from fluorochromes into secondary fluorescence detectors of a flow cytometer. Flow cytometers are designed to have a primary detector for each fluorochrome label (e.g. FL1- FITC, FL2- PE, FL3- PE-Cy™5). Fluorescent signals emitted by fluorochromes can bleed or overlap into the secondary fluorescence detectors.

    The Simply Cellular® Compensation Standard is a mixture of 2 populations of microspheres that have the ability to bind human monoclonal antibodies at high and low capacities, respectively. The microspheres are supplied in a sterile-filtered, pH buffered PBS solution containing surfactant and preservatives. As the operator labels these standards with the same antibody used to label the cell samples, the standards will exhibit spectral properties that closely match the cells being analyzed.

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  46. Flow Cytometry Protein A Antibody Binding Beads

    Flow Cytometry Protein A Antibody Binding Beads

    Starting at: €280.00

    Single population Protein A microspheres are suitable for labeling with conjugated antibodies from a range of hosts. Labeled microspheres may be used as single-population reference standards or in conjuction with an unlabeled population for compensation purposes.

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  47. Flow Cytometry Protein G Antibody Binding Beads

    Flow Cytometry Protein G Antibody Binding Beads

    Starting at: €280.00

    Single population Protein G microspheres are suitable for labeling with conjugated antibodies from a range of hosts. Labeled microspheres may be used as single-population reference standards or in conjuction with an unlabeled population for compensation purposes.

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  48. FITC/PE Compensation Standard | Polysciences, Inc.

    FITC/PE Compensation Standard

    Starting at: €220.00

    The FITC/PE Compensation Standard is a convenient means of setting two color compensation on a flow cytometer. Each kit contains an Autofluor™ bead, as well as a bead labeled with FITC, a bead labeled with PE, and one labeled with both FITC and PE.

    A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

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  49. Simply Cellular® anti-Mouse for Violet Laser

    Simply Cellular® anti-Mouse for Violet Laser

    Starting at: €266.00

    The Simply Cellular® anti-Mouse for Violet Laser standard features microspheres comprised of a proprietary matrix that exhibits low autofluorescence with violet excitation. Beads are suitable for labeling with mouse Abs conjugated with violet fluorochromes, and for use as a compensation or general reference standard for detectors off of the violet laser. Beads are also suitable for use with other fluorochromes / detectors, e.g. 488nm, 633nm. They are supplied in aqueous suspension containing ProClin®.

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  50. Viability Dye Compensation Standard, 4µm

    Viability Dye Compensation Standard, 4µm

    Starting at: €343.00

    Viability Dye Compensation Standards are suitable for labeling with LIVE/DEAD® stains or similarly-reactive dyes to generate compensation standards for flow cytometric analyses. Beads are not suitable for labeling with DNA stains such as propidium iodide, DAPI, or SYTOX®, and users should contact us for discussion if uncertain as to the compatability of a specific dye or stain.

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