The PolyLink Amine Linker Kit with Columns and Reagents for Enzyme Immobilization includes 1 gram each of 4 different Purolite® amine functionalized resins along with all of the necessary Polysciences glutaraldehyde and buffer solution needed to carry out a standard coupling protocol to bond your enzyme to the resins. The resins are individually packaged in ready-to-use 10 mL columns with a bottom frit to allow you to rapidly treat the resins with reagents, filter off excess reagents, and rinse, all in one vessel. The total working volume of the columns is 22 mL when the column head-space is included.
The included resins have 2 different linker lengths and 2 different porosities to allow you to rapidly identify the best resin for immobilizing your enzyme. Amino resins can be used to covalently immobilize enzymes, following preactivation using glutaraldehyde, as shown in the figure. Reaction of the aldehyde groups with amino groups of enzymes to form Schiff bases is fast and gives stable multipoint covalent binding. If desired, the imine linkages can be made even more stable by reducing the double bonds using a borohydride or another compatible reducing agent (reducing agent is not included in this kit).
For more details download the Product Guide
|Product ID||Description||Functional Group||Immobilization||Surface Area||Pore Diameter||Water Content|
|50275A||ECR8310F Amino C2 acrylate (1 g in a 10 mL column)||NH2 (short spacer)||Covalent||> 70||850 - 1200||58 - 62|
|50275B||ECR8319F Amino C2 acrylate (1 g in a 10 mL column)||NH2 (short spacer)||Covalent||> 50||1600 - 2000||62 - 66|
|50275C||ECR8405F Amino C2 acrylate (1 g in a 10 mL column)||NH2 (short spacer)||Covalent||> 40||450 - 850||50 - 55|
|50275D||ECR8417F Amino C2 acrylate (1 g in a 10 mL column)||NH2 (short spacer)||Covalent||> 50||1600 - 2200||61 - 65|
|Kit Components||Description||Number Included|
|01909A||Glutaraldehyde 25% in Ampules (10 mL)||2|
|19540A-225||Phosphate buffered saline (225 mL)||2|
|19540B||8% Glutaraldehyde in PBS (empty labeled bottle)||