Filipin (from Streptomyces filipinensis)
Filipin is highly fluorescent polyene macrolide that binds specifically to cholesterol. It is widely used as a histochemical fluorescent stain for cholesterol. This method of detecting cholesterol in cell membranes is used clinically in the study and diagnosis of Type C Niemann-Pick disease. (This product is for research use only)
Filipin is a polyene antifungal antibiotic produced naturally by the bacteria Streptomyces filipinensis.
Maekawa, M. & Fairn, G. D. Complementary probes reveal that phosphatidylserine is required for the proper transbilayer distribution of cholesterol. Journal of Cell Science 128, 1422–1433 (2015). doi:10.1242/jcs.164715
Tamura, A. & Yui, N. Lysosomal-specific cholesterol reduction by biocleavable polyrotaxanes for ameliorating Niemann-Pick type C disease. Scientific reports 4, 4356 (2014). doi:10.1038/srep04356
Arthur, J. R., Heinecke, K. A. & Seyfried, T. N. Filipin recognizes both GM1 and cholesterol in GM1 gangliosidosis mouse brain. Journal of lipid research 52, 1345–51 (2011). doi:10.1194/jlr.M012633
Kruth, H. S., Cupp, J. E. & Khan, M. A. Method for detection and isolation of cholesteryl ester-containing ‘foam’ cells using flow cytometry. Cytometry 8, 146–152 (1987). doi:10.1002/cyto.990080207
Skin sensitizer
