NeuroVue® Red Solid - For Neuronal Tract Tracing

NeuroVue^® Red Solid can be formulated as desired for applications in which the filter format is not suitable. NeuroVue^® Red Solid is useful for neuronal tract tracing studies of up to 3-4 weeks. Spectrally compatible with eGFP, YFP in many systems, and NeuroVue^® Maroon & Jade. Spectral unmixing required for use with NeuroVue^® Orange.

NeuroVue^® Red Solid is available in solid form for researchers who would like to make their own dye-coated materials for application to tissue (e.g. use for coating glass/metal pins or pieces of hair to provide a more rigid support when smaller injections are required). 1 mg of solid dye.

Advantages:

• Diffusion properties comparable to or better than other commercially available neurotracing dyes
• Can be formulated as desired for application
• More focal results (e.g. labeling of small sets of axons within a pathway)
• Available in multiple colors, including far red, for multi-tract tracing and improved results even in tissues with high myelin expression

Applications:

NeuroVue^® Red has been found to be useful for tracing neuronal connections in animal tissues fixed in formaldehyde (1, 3-6, 8, 10, 12, 13). Like other lipophilic tracers (7, 9), it readily transfers into plasma membranes in fixed and/or live tissues and diffuses laterally within the membrane, eventually labeling the entire cell body as well as the finest axonal and dendritic branches, and allowing visualization of neuronal processes up to several millimeters distant from the point of dye insertion (1, 3-6, 8, 10, 12, 13).

NeuroVue Red fluoresces in the red and exhibits minimal bleed through into filter windows typically used for green fluorescing lipophilic tracers such as NeuroVue Jade and far red fluorescing lipophilic tracers such as NeuroVue Maroon or NeuroVue Burgundy, making it an excellent choice for multi-color neural tracing studies in sections and/or whole mount preparations (1, 3-6, 8, 10, 12, 13). In addition, NeuroVue Red can be used in combination with NeuroVue^® Orange if spectral unmixing techniques are employed.

Publications:

• Diffusion and Imaging Properties of three new lipophilic tracers, NeuroVue^® Maroon, NeuroVue^® Red and NeuroVue^® Green and their use for double and triple labeling of neuronal profile. B Fritzsch et al. Brain Res Bull., 66, 3, 249-258 (2005).
• A disorganized innervation of the inner ear persists in the absence of ErbB2. JK Morris et al. Brain Res.,1091:186-199 (2006).
• Deafferentiation induces novel projections in the auditory brainstem after hearing onset. CY Hsieh and KS Cramer. Journal of Comparative Neurology, 497:589-599 (2006)
• Al-Mehdi AB, Patel M, Haroon A, Reed D, Ohlsson-Wilhelm B, Muirhead K and Gray BD. Increased Depth of Cellular Imaging in the Intact Lung using Far Red and Near Infrared Fluorescent Probes. Int. J. Biomed Imaging. 1-7 (2006).
• Stewart CC, Woodring ML, Podniesinski E and Gray BD. Flow cytometer in the infrared: inexpensive modifications to a commercial instrument. Cytometry, Part A, 67A, #2, 104-111, 2005

NeuroVue is a registered trademark of PTI Research, Inc. For patents see list.