Specific conductance of 35% (w/v) solution 2µmho/cm. Used in electrophoresis for separation of nucleic acid fragments and proteins. For precision gels used in electro-phoresis and isoelectric focusing. Because of the low acidity levels of our acrylamide, binding of ampholytes is virtually eliminated and fine pH gradients can be established. Fluorescence-free gels are readily prepared. For scanning of gels n the UV region, our Ultra Pure grade acrylamide makes gels with baselines having very low "noise".

>99.9%

Dissolve in distilled or deionized water heated to 60°C, under a hood.

(Sodium cacodylate)

Used in combination with methyl green for the selective and differential staining of nucleic acids. The pyronin Y stains RNA red, while the methyl green stains DNA green. The combined methyl green-pyronin Y stain is a useful histochemical reagent. Pyronin Y can also be used as a tracking dye for polyacrylamide gel electrophoresis.

λmax 552nm

Equal to or better than ACS specifications. Silver stain techniques are widely used to detect nanogram quantities of proteins following electrophoresis. Silver nitrate is the silver source in most silver stain procedures.

A post-electrophoresis stain for lipoproteins on cellulose acetate plates.

(Solvent red 27; Sudan red 5B; Ceres red 5B)

(Food green 3). Sensitive stain for proteins in polyacrylamide gels. Especially suitable in isoelectric focusing. Also suitable for use as a cytological counterstain, and mammalian tissue stain for muscle and collagen.

λ max 622-626nm.

Protein stain for SDS gels. Used in dye binding techniques for protein quantification.

(Coomassie blue® G250; Brilliant blue G250; Acid blue 90)

λmax 610nm

Rapid acting and sensitive dye for SDS gels.

(Coomassie blue® R250; Brilliant blue R250; Acid blue 83)

λmax 588nm

Buffer for fixatives for light and electron microscopy; e.g., osmium tetroxide, formaldehyde, glutaraldehyde, Karnovsky’s fixative.

Silver staining is a highly sensitive method for detecting proteins in polyacrylamide slab gels. Most silver staining protocols are time consuming, complicated, and dependent upon the purity of the reagents. Polysciences’ Silver Stain kit is simple, stable, controllable, and very rapid. Our method is sensitive to proteins in the nanogram range and may be used either before or after Coomassie blue staining. Staining for proteins is initiated in an alkali environment.

Used primarily for demonstrating acid mucopolysaccarides with Scott's method and Mowry's staining methods. Used in electrophoresis for detecting glycoproteins.

Glycoproteins are macromolecules composed of both a protein and carbohydrate portion. The sugar group can assist in protein folding or improved stability.

Glycoproteins are often found in proteins that are located in extracellular spaces and are important in immune cell recognition such as: antibodies and Major Histocompatibility Complex (MHC).