Single population Protein A microspheres are suitable for labeling with conjugated antibodies from a range of hosts. Labeled microspheres may be used as single-population reference standards or in conjuction with an unlabeled population for compensation purposes.

Single population Protein G microspheres are suitable for labeling with conjugated antibodies from a range of hosts. Labeled microspheres may be used as single-population reference standards or in conjuction with an unlabeled population for compensation purposes.

Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Supplied as 2 x 7ml.

Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

Each standard consists of a population of microspheres surface labeled with a single fluorochrome at a high intensity level. The Right Reference Standard™ microspheres approximate the size of human lymphocytes (~7-9µm). With their narrow fluorescence CVs, Right Reference Standard™ products are suitable for alignment purposes. These standards may also be used to QC a specific path of the optical system (laser/filter/PMT), and to establish a unified fluorescence range for a particular detector.

Each standard consists of a population of microspheres surface labeled with a single fluorochrome at a high intensity level. The Right Reference Standard™ microspheres approximate the size of human lymphocytes (~7-9µm). With their narrow fluorescence CVs, Right Reference Standard™ products are suitable for alignment purposes. These standards may also be used to QC a specific path of the optical system (laser/filter/PMT), and to establish a unified fluorescence range for a particular detector.

Each standard consists of a population of microspheres surface labeled with a single fluorochrome at a high intensity level. The Right Reference Standard™ microspheres approximate the size of human lymphocytes (~7-9µm). With their narrow fluorescence CVs, Right Reference Standard™ products are suitable for alignment purposes. These standards may also be used to QC a specific path of the optical system (laser/filter/PMT), and to establish a unified fluorescence range for a particular detector.

Each standard consists of a population of microspheres surface labeled with a single fluorochrome at a high intensity level. The Right Reference Standard™ microspheres approximate the size of human lymphocytes (~7-9µm). With their narrow fluorescence CVs, Right Reference Standard™ products are suitable for alignment purposes. These standards may also be used to QC a specific path of the optical system (laser/filter/PMT), and to establish a unified fluorescence range for a particular detector.

The Simply Cellular® anti-Mouse Compensation Standard is a mixture of two Simply Cellular® anti-Mouse IgG particle populations capable of binding high and low levels of mouse monoclonal antibody used in your assay. Ideal for performing compensation in multicolor (2, 3, 4, or more) analysis.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

The Simply Cellular® anti-Rat Compensation Standard is a mixture of two Simply Cellular® anti-Rat IgG particle populations capable of binding high and low levels of rat monoclonal antibody used in your assay. Ideal for performing compensation in multicolor (2, 3, 4, or more) analysis.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Flow cytometry is a highly accurate technology used for cell measurements. High quality particles, supplied from Polysciences, are used to calibrate these instruments.

This product is a component of the Flow Check™ YG 6.0 Kit (Cat. #23512), which allows comparision of various levels of green fluorescence. The full kit contains 4 components, each of which is packaged with ~2 x 10⁶ particles per ml: a full intensity bead, two intermediate intensity beads and a blank bead.

Polysciences offers a series of monodisperse, low CV particles with two different dyes in sizes with nominal diameters ranging from 0.50µm to 6.0µm. All particles have been screened by flow cytometry labs to be suitable for instrument alignment. We use our own proprietary dyes to ensure long term stability.

Flow cytometry is a highly accurate technology used for cell measurements. High quality particles, supplied from Polysciences, are used to calibrate these instruments.

This product is a component of the Flow Check™ YG 6.0 Kit (Cat. #23512), which allows comparision of various levels of green fluorescence. The full kit contains 4 components, each of which is packaged with ~2 x 10⁶ particles per ml: a full intensity bead, two intermediate intensity beads and a blank bead.

Flow cytometry is a highly accurate technology used for cell measurements. High quality particles, supplied from Polysciences, are used to calibrate these instruments.

This product is a component of the Flow Check™ YG 6.0 Kit (Cat. #23512), which allows comparision of various levels of green fluorescence. The full kit contains 4 components, each of which is packaged with ~2 x 10⁶ particles per ml: a full intensity bead, two intermediate intensity beads and a blank bead.

The particles in this kit allow comparision of various levels of green fluorescence. Kit contains 4 components, each packaged with ~2 x 10⁶ particles per ml: a full intensity bead, two intermediate intensity beads and a blank bead.

Flow Check™Ruby Red Fluorescent Microspheres are ~6µm narrow distribution polystyrene particles that can be used for flow cytometry applications where fluorescence emission in the range of 600nm to 710nm is needed. Suitable for 488nm and 663nm excitation, detection in PE-Cy5 or APC channels. Supplied as a 5ml suspension (~5 x 10⁶ beads/ml) in convenient dropper bottle.

• Excitation maximum: 475nm
• Emission maximum: 663nm

Our flow cytometry customers demand precisely manufactured microparticles as calibration tools. Our unique synthesis methods routinely result in particles with fluorescence CV’s less than 5% and often as low as 1%. This kit guarantees that our discriminating customer will have the finest particles with the lowest fluorescence CV currently available from our stock.

Quantitative MESF Kits consist of a series of reference microbead standards whose intensities have been calibrated in Molecules of Equivalent Soluble Fluorochrome (MESF) units. Our FREE Flow Cytometry Data Analysis template, QuickCal®, works with all Quantum™ MESF Kits (access QuickCal® by visiting www.bangslabs.com/products/quickcal and entering the Access Number provided with your standards).

Quantum™ FITC-5 MESF kits include six microsphere populations that are ~7-9µm in diameter: one blank and five labeled with increasing amounts of fluorochrome. The assignment of fluorescence intensity in MESF units is performed through direct comparison of fluorescence measurements from solutions of the pure fluorochrome with those from microspheres surface-labeled with the same fluorochrome. In addition to quantitative fluorescence cytometry, Quantum™ MESF kits may be used to assess the detection threshold, resolution, and linearity of the instrument. QuickCal® is provided with each kit to aid in determining the expression level of cells, and for evaluating instrument linearity and detection threshold.

Quantum™ FITC-5 MESF kits include six microsphere populations that are ~7-9µm in diameter: one blank and five labeled with increasing amounts of fluorochrome. The assignment of fluorescence intensity in MESF units is performed through direct comparison of fluorescence measurements from solutions of the pure fluorochrome with those from microspheres surface-labeled with the same fluorochrome. In addition to quantitative fluorescence cytometry, Quantum™ MESF kits may be used to assess the detection threshold, resolution, and linearity of the instrument. QuickCal® is provided with each kit to aid in determining the expression level of cells, and for evaluating instrument linearity and detection threshold.

Ultra Rainbow Fluorescent Particles are internally labeled with a mixture of fluorophores which enables them to be excited at wavelengths from 365nm to 650nm.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter to approximate the size of human lymphocytes.

Ultra Rainbow Fluorescent Particles are internally labeled with a mixture of fluorophores which enables them to be excited at wavelengths from 365nm to 650nm.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter to approximate the size of human lymphocytes.

Antibody Binding Standards are particles which have ligands covalently linked to their surfaces to bind specific proteins (e.g. Mouse IgG antibodies) in calibrated quantities.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Antibody Binding Standards are particles which have ligands covalently linked to their surfaces to bind specific proteins (e.g. Human IgG antibodies) in calibrated quantities.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Antibody Binding Standards are particles which have ligands covalently linked to their surfaces to bind specific proteins (e.g. Human IgG antibodies) in calibrated quantities.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Quantum™ Simply Cellular® Kits contain a mixture of microbeads which bind specific amounts of IgG antibodies. These standards are calibrated in terms of Antibody Binding Capacity (ABC) and can be used to construct calibration plots to determine the ABC of unknown samples and monitor instrument performance. Our FREE Flow Cytometry Data Analysis template, QuickCal®, is designed to work with all Quantum™ Simply Cellular® Kits (access QuickCal® by visiting www.bangslabs.com/products/quickcal and entering the Access Number provided with your standards).

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Quantum™ Simply Cellular® Kits contain a mixture of microbeads which bind specific amounts of IgG antibodies. These standards are calibrated in terms of Antibody Binding Capacity (ABC) and can be used to construct calibration plots to determine the ABC of unknown samples and monitor instrument performance. Our FREE Flow Cytometry Data Analysis template, QuickCal®, is designed to work with all Quantum™ Simply Cellular® Kits (access QuickCal® by visiting www.bangslabs.com/products/quickcal and entering the Access Number provided with your standards).

The FITC/PE Compensation Standard is a convenient means of setting two color compensation on a flow cytometer. Each kit contains an Autofluor™ bead, as well as a bead labeled with FITC, a bead labeled with PE, and one labeled with both FITC and PE.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Quantitative MESF Kits consist of a series of reference microbead standards whose intensities have been calibrated in Molecules of Equivalent Soluble Fluorochrome (MESF) units. Our FREE Flow Cytometry Data Analysis template, QuickCal®, works with all Quantum™ MESF Kits (access QuickCal® by visiting www.bangslabs.com/products/quickcal and entering the Access Number provided with your standards).

A test requires one drop (50μl, ~100,000 particles) of each particle suspension. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Quantitative MESF Kits consist of a series of reference microbead standards whose intensities have been calibrated in Molecules of Equivalent Soluble Fluorochrome (MESF) units. Our FREE Flow Cytometry Data Analysis template, QuickCal®, works with all Quantum™ MESF Kits (access QuickCal® by visiting www.bangslabs.com/products/quickcal and entering the Access Number provided with your standards).

A test requires one drop (50μl, ~100,000 particles) of each particle suspension. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Size Calibration Standards include five highly uniform unlabeled microbead populations of different diameters (~4-11µm), which can be used to construct calibration plots for electronic volume and light scatter instrumentation.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Our Time Delay Calibration Standard is intended for use in assessing the delay between blue and red lasers. It features ~6µm microspheres dyed with a fluorophore that is excited with 488nm or 635nm excitation, and exhibits red / far-red emission.

The Simply Cellular® anti-Mouse for Violet Laser standard features microspheres comprised of a proprietary matrix that exhibits low autofluorescence with violet excitation. Beads are suitable for labeling with mouse Abs conjugated with violet fluorochromes, and for use as a compensation or general reference standard for detectors off of the violet laser. Beads are also suitable for use with other fluorochromes / detectors, e.g. 488nm, 633nm. They are supplied in aqueous suspension containing ProClin®.

Multi-color Reference Standards are highly uniform microspheres which fluoresce over a broad range of the spectrum to provide several reference positions in multi-detector analysis. This standard is used to properly align your flow cytometer excited from UV to near IR; this standard emits over the entire spectrum.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter to approximate the size of human lymphocytes.

Single-color Fluorescence Reference Standards are labeled with specific fluorochromes to exhibit the same spectral characteristics as labeled cells. Their size approximates that of human lymphocytes (~7-9µm in diameter) and are available in a wide vareity of fluorescent dyes. Fluorescein is also available as a 2µm bead. These standards may be used to QC a specific path of the optical system (laser/filter/PMT), to optimize filter and mirror sets for fluorophores, and to establish a test-specific Target Channel Value for instrument set-up.

Single-color Fluorescence Reference Standards are labeled with specific fluorochromes to exhibit the same spectral characteristics as labeled cells. Their size approximates that of human lymphocytes (~7-9µm in diameter) and are available in a wide vareity of fluorescent dyes. Fluorescein is also available as a 2µm bead. These standards may be used to QC a specific path of the optical system (laser/filter/PMT), to optimize filter and mirror sets for fluorophores, and to establish a test-specific Target Channel Value for instrument set-up.

Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

Fluorescence Reference Standards are labeled with specific fluorochromes so that they give rise to the same fluorescence spectra as cells labeled with the same fluorochromes. Fluorescence intensity is similar to biological samples.

Current applications in flow cytometry extend far beyond traditional lymphocyte immunophenotyping, with some involving the analysis of very small particles such as platelet- and endothelial-derived microparticles or microbial species. Our Small Bead Calibration Kits allow operators to verify the resolution capabilities of the flow cytometer, and to establish appropriate instrument settings for the analyses.

Nominal Diameters: 0.2μm, 0.5μm, 0.8μm

Particle Concentration: 1 x 10⁸ particles/ml

Fluorescence: YG (488nm excitation, green emission)

Current applications in flow cytometry extend far beyond traditional lymphocyte immunophenotyping, with some involving the analysis of very small particles such as platelet- and endothelial-derived microparticles or microbial species. Our Small Bead Calibration Kits allow operators to verify the resolution capabilities of the flow cytometer, and to establish appropriate instrument settings for the analyses.

Nominal Diameters: 1.0μm, 3.0μm, 6.0μm

Particle Concentration: 2 x 10⁶ particles/ml

Fluorescence: YG (488nm excitation, green emission)

Viability Dye Compensation Standards are suitable for labeling with LIVE/DEAD® stains or similarly-reactive dyes to generate compensation standards for flow cytometric analyses. Beads are not suitable for labeling with DNA stains such as propidium iodide, DAPI, or SYTOX®, and users should contact us for discussion if uncertain as to the compatability of a specific dye or stain.

Viability Dye Compensation Standards are suitable for labeling with LIVE/DEAD® stains or similarly-reactive dyes to generate compensation standards for flow cytometric analyses. Beads are not suitable for labeling with DNA stains such as propidium iodide, DAPI, or SYTOX®, and users should contact us for discussion if uncertain as to the compatability of a specific dye or stain.

Polysciences offers a series of monodisperse, low CV particles with two different dyes in sizes ranging from 0.50µm to 6.0µm. All particles have been screened by flow cytometry labs to be suitable for instrument alignment. We use our own proprietary dyes to ensure long term stability.

Component of the Flow Check™ YG Size Range Calibration Kit (Cat. #23514)

Polysciences offers a series of monodisperse, low CV particles with two different dyes in sizes ranging from 0.50µm to 6.0µm. All particles have been screened by flow cytometry labs to be suitable for instrument alignment. We use our own proprietary dyes to ensure long term stability.

Component of the Flow Check™ YG Size Range Calibration Kit (Cat. #23514)

Polysciences offers a series of monodisperse, low CV particles with two different dyes in sizes ranging from 0.50µm to 6.0µm. All particles have been screened by flow cytometry labs to be suitable for instrument alignment. We use our own proprietary dyes to ensure long term stability.

Component of the Flow Check™ YG Size Range Calibration Kit (Cat. #23514)

The Flow Cytometry Absolute Count Standard™ consists of highly uniform cell-sized microspheres labeled with a full spectrum dye, and provided at a known concentration. These beads fluoresce in FL1, FL2, and FL3, allowing them to be used as count standards in all three channels.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Quantum™ QC is a multi-intensity, multi-fluorescent standard that is intended for use as an in-depth tool for daily cytometer QC, and is appropriate for use with all lasers and detectors.

Used in conjunction with hardware or software to remove spectral overlap from fluorochromes into secondary fluorescence detectors of a flow cytometer. Flow cytometers are designed to have a primary detector for each fluorochrome label (e.g. FL1- FITC, FL2- PE, FL3- PE-Cy™5). Fluorescent signals emitted by fluorochromes can bleed or overlap into the secondary fluorescence detectors.

The Simply Cellular® Compensation Standard is a mixture of 2 populations of microspheres that have the ability to bind human monoclonal antibodies at high and low capacities, respectively. The microspheres are supplied in a sterile-filtered, pH buffered PBS solution containing surfactant and preservatives. As the operator labels these standards with the same antibody used to label the cell samples, the standards will exhibit spectral properties that closely match the cells being analyzed.

The Nanobead Calibration Kit comprises highly defined microspheres with an internalized fluorescent dye to allow users to determine the capabilities of their cytometer and appropriate instruments settings, as size references for small particle characterization, and assessing small particle handling processes.

Quantum™ Simply Cellular® Kits contain a mixture of microbeads which bind specific amounts of IgG antibodies. These standards are calibrated in terms of Antibody Binding Capacity (ABC) and can be used to construct calibration plots to determine the ABC of unknown samples and monitor instrument performance. Our FREE Flow Cytometry Data Analysis template, QuickCal®, is designed to work with all Quantum™ Simply Cellular® Kits (access QuickCal® by visiting www.bangslabs.com/products/quickcal and entering the Access Number provided with your standards).

Quantitative MESF Kits consist of a series of reference microbead standards whose intensities have been calibrated in Molecules of Equivalent Soluble Fluorochrome (MESF) units. Our FREE Flow Cytometry Data Analysis template, QuickCal®, works with all Quantum™ MESF Kits (access QuickCal® by visiting www.bangslabs.com/products/quickcal and entering the Access Number provided with your standards).