Acid Fuchsin is one of the dyes used in the Masson's Trichrome Staining technique. This method is commonly used to stain tissue sections in the Histology and Cytology Laboratory in order to distinguish muscle from collagen. The muscle stains red with the acid fuchsin and the collagen is stained green or blue with light green SF yellowish or methyl blue.

Used in combination with methyl green for the selective and differential staining of nucleic acids. The pyronin Y stains RNA red, while the methyl green stains DNA green. The combined methyl green-pyronin Y stain is a useful histochemical reagent. Pyronin Y can also be used as a tracking dye for polyacrylamide gel electrophoresis.

λmax 552nm

DNA intercalating dye. A grade of acridine orange of exceptionally high purity, suitable for quantitative work. Free of inorganic salts. A specific stain for RNA, used as a 2% solution containing 1% lanthanum acetate in 15% acetic acid.

A post-electrophoresis stain for lipoproteins on cellulose acetate plates.

(Solvent red 27; Sudan red 5B; Ceres red 5B)

Cardiac muscle stain.

(Direct red 80)

(Food green 3). Sensitive stain for proteins in polyacrylamide gels. Especially suitable in isoelectric focusing. Also suitable for use as a cytological counterstain, and mammalian tissue stain for muscle and collagen.

λ max 622-626nm.

Protein stain for SDS gels. Used in dye binding techniques for protein quantification.

(Coomassie blue® G250; Brilliant blue G250; Acid blue 90)

λmax 610nm

Rapid acting and sensitive dye for SDS gels.

(Coomassie blue® R250; Brilliant blue R250; Acid blue 83)

λmax 588nm

Nuclear protein stain and glycogen stain. Also a general tissue stain for human, animal and VIR histology.

Electron dense metal stain.

(12-Molybdophosphoric acid)

Useful for staining blood films and malarial parasites in blood films.

For staining bacilli in tissue. Coupling agent for ultrastructural localization of esterases.

(Pararosaniline hydrochloride; Basic red 9) λ max 542-548nm

A general biological stain. Used as a nuclear stain for histological studies. Also used as a cationic lipophilic probe and in the detection of glycosaminoglycans and proteoglycans.

(Basic red 2; 3,7-Diamino-2,8-dimethyl-5-phenylphenazinium chloride) λ max 530nm.

Widely used as a counterstain. Also, useful plasma stain.

(acid red 66; Ponceau B) λ max 505nm

Used as contrast stain in histology and cytology and as a pH indicator (pH 10.0-13.0). Used with acid fuchsin as Mallory’s connective tissue stain. Also used to visualize chromosomes and cellulose wall implants. (Acid blue 22).

Used as a dye and as a biological stain. Certified for use as a counterstain in cytology. An important contrast stain for plasma as a critical component of Papanicolaou (PAP) stains. Stains collagen fibers when substituted for aniline blue in Masson’s trichrome.

λ max 630(422)nm

A valuable stain in general histology and cytology. Gives sharp, clear-cut pictures of both nuclei and cytoplasmic structures. Also useful for differentiation of fungi.

Nuclear fast red staining is a simple method of nuclear chromatin staining and is mainly used for high-contrast counterstaining for histological applications.

Used primarily for demonstrating acid mucopolysaccarides with Scott's method and Mowry's staining methods. Used in electrophoresis for detecting glycoproteins.

(9-Amino-5-imino-5H-benzo[a]phenoxazine acetate salt) Cresyl Violet Acetate is a certified solid dye used for the preparation of a staining solution to demonstrate the presence of Nissl substances in neurons and cell nuclei.

Sudan Black B is useful for staining neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections.

Mallory’s stain for collagen in connective tissue. Also useful as a stain for granules, elastic fibers, mast cells, and pollen tubes. A general histology and cytology counterstain.

Acid orange 10; Xylene fast orange G; 7-Hydroxy-8-phenylazo-1,3-naphthalenedisulfonic acid λmax 475nm.

Cytoplasmic counterstain. Component of Wright stain and Tetrachrome stain.

(Eosin yellowish; Acid red 87; 2’,4’,5’,7’-Tetrabromo-fluorescein, disodium salt)

λmax: 517nm

Anionic metachromatic dye. Good contrast stain or counterstain. Specific stain for amyloids in pathology. Also, a pH indicator; transition interval: pH 3.0 (blue) to 5.0 (red).

λ max: 497nm

Hydroxynapthol Blue is suitable for calcium determinations.

A metachromatic, cationic thiazine dye that is widely used in in vitro biological applications. It has also been used in techniques for DNAase detection.

λmax 626nm

Useful for staining RNA, oligodeoxynucleotides, proteins and glycosaminoglycans.

λ max 626nm.

Assay >60% anhydrous dye.

(Taylor’s blue; 3,7’-Bis(dimethylamino)-1,9-dimethyldiphenothiazin-5-ium chloride) λ max 649nm

Used in dye binding assays for glycosaminoglycans.