DODAP (1,2-dioleoyl-3-dimethylammonium propane)
C41H77NO4
- Key Features
- High transfection efficiency and low cytotoxicity
- Efficient delivery of plasmid DNA, siRNA/miRNA, and CRISPR/Cas9 components
- Widely used in nanomedicine
- Flexible and scalable
DODAP (1,2-dioleoyl-3-dimethylammonium-propane) is an ionizable cationic lipid with lower cytotoxicity and high transfection efficiency. DODAP is neutral at physiological pH, but acquires a positive charge inside the endosome due to the protonation of free amines when pH is lower than its pKa (<7). The electrostatic interactions between DODAP and naturally occurring anionic lipids in endosomal membranes trigger the release of nucleic acid. These interactions promote membrane lytic non-bilayer structures to enable the intracellular delivery of nucleic acid. A common application is in nanomedicine as an ionizable lipid component of nanocarriers (lipid-polymer hybrid nanoparticles, LPNs), widely used to encapsulate bioactive molecules, including mRNA, siRNA, and plasmid DNA as a treatment for diseases.
Contact us at [email protected] to know about our cGMP grade DODAP manufactured under 21 CFR part 210,211.
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