Silver staining is a highly sensitive method for detecting proteins in polyacrylamide slab gels. Most silver staining protocols are time consuming, complicated, and dependent upon the purity of the reagents. Polysciences’ Silver Stain kit is simple, stable, controllable, and very rapid. Our method is sensitive to proteins in the nanogram range and may be used either before or after Coomassie blue staining. Staining for proteins is initiated in an alkali environment.

Von Kossa Method for Calcium Kit demonstrates only calcium phosphate and calcium carbonate salts. Note that when used in skin specimens that the silver will reduce the melanin in the skin, to also give a black deposit.

Chromic acid oxidation forms aldehydes from fungal cell wall polysaccharide components, which are subsequently demonstrated by reduction of an alkaline hexamine-silver complex. The reaction may be compared to that of the periodic acid Schiff reaction.

Intended for the use in the histological visualization of Argentaffin cells and Melanin in paraffin or frozen sections.

Equal to or better than ACS specifications. Silver stain techniques are widely used to detect nanogram quantities of proteins following electrophoresis. Silver nitrate is the silver source in most silver stain procedures.

Periodic Acid Schiff Methenamine Silver Stain for Basement Membranes

A new and improved Jones PAS-M technique that utilizes an enhancer to accelerate the methenamine silver reaction with the glomerular basement membranes, while reducing the time to achieve results better than the original Jones PAS-M and a more stabilized silver solution. Used in kidney biopsies, Jones PAS-M may also be used for plastic sections for glomerular and tubular basement membranes.

Since plastic sections have a tendency to come off slides in staining procedures that require heat we recommend using Tissue Tack Microscope Slides Cat. #24216

Identifies reticulin fibers in tissue sections liver, kidney and spleen. Reticulin is a type III collagen found in the basement membrane of many organs and provides structural integrity.