Size Calibration Standards include five highly uniform unlabeled microbead populations of different diameters (~4-11µm), which can be used to construct calibration plots for electronic volume and light scatter instrumentation.

A test requires one drop (50μl) of particle suspension, which is equivalent to ~100,000 particles. Bangs Flow Cytometry Standards are 7-9μm in diameter (unless otherwise noted) to approximate the size of human lymphocytes.

Current applications in flow cytometry extend far beyond traditional lymphocyte immunophenotyping, with some involving the analysis of very small particles such as platelet- and endothelial-derived microparticles or microbial species. Our Small Bead Calibration Kits allow operators to verify the resolution capabilities of the flow cytometer, and to establish appropriate instrument settings for the analyses.

Nominal Diameters: 0.2μm, 0.5μm, 0.8μm

Particle Concentration: 1 x 10⁸ particles/ml

Fluorescence: YG (488nm excitation, green emission)

Current applications in flow cytometry extend far beyond traditional lymphocyte immunophenotyping, with some involving the analysis of very small particles such as platelet- and endothelial-derived microparticles or microbial species. Our Small Bead Calibration Kits allow operators to verify the resolution capabilities of the flow cytometer, and to establish appropriate instrument settings for the analyses.

Nominal Diameters: 1.0μm, 3.0μm, 6.0μm

Particle Concentration: 2 x 10⁶ particles/ml

Fluorescence: YG (488nm excitation, green emission)

The Nanobead Calibration Kit comprises highly defined microspheres with an internalized fluorescent dye to allow users to determine the capabilities of their cytometer and appropriate instruments settings, as size references for small particle characterization, and assessing small particle handling processes.