For staining bacilli in tissue. Coupling agent for ultrastructural localization of esterases.

(Pararosaniline hydrochloride; Basic red 9) λ max 542-548nm

A general biological stain. Used as a nuclear stain for histological studies. Also used as a cationic lipophilic probe and in the detection of glycosaminoglycans and proteoglycans.

(Basic red 2; 3,7-Diamino-2,8-dimethyl-5-phenylphenazinium chloride) λ max 530nm.

Widely used as a counterstain. Also, useful plasma stain.

(acid red 66; Ponceau B) λ max 505nm

Used as contrast stain in histology and cytology and as a pH indicator (pH 10.0-13.0). Used with acid fuchsin as Mallory’s connective tissue stain. Also used to visualize chromosomes and cellulose wall implants. (Acid blue 22).

Used as a dye and as a biological stain. Certified for use as a counterstain in cytology. An important contrast stain for plasma as a critical component of Papanicolaou (PAP) stains. Stains collagen fibers when substituted for aniline blue in Masson’s trichrome.

λ max 630(422)nm

A valuable stain in general histology and cytology. Gives sharp, clear-cut pictures of both nuclei and cytoplasmic structures. Also useful for differentiation of fungi.

Nuclear fast red staining is a simple method of nuclear chromatin staining and is mainly used for high-contrast counterstaining for histological applications.

Used primarily for demonstrating acid mucopolysaccarides with Scott's method and Mowry's staining methods. Used in electrophoresis for detecting glycoproteins.

Sudan Black B is useful for staining neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections.

Mallory’s stain for collagen in connective tissue. Also useful as a stain for granules, elastic fibers, mast cells, and pollen tubes. A general histology and cytology counterstain.

Acid orange 10; Xylene fast orange G; 7-Hydroxy-8-phenylazo-1,3-naphthalenedisulfonic acid λmax 475nm.

Anionic metachromatic dye. Good contrast stain or counterstain. Specific stain for amyloids in pathology. Also, a pH indicator; transition interval: pH 3.0 (blue) to 5.0 (red).

λ max: 497nm

Demonstrates neutral and acidic mucosubstances on tissue at pH 2.5 imparts a blue color to the acidic mucins and other carboxylated or weakly sulphated acid mucosubstances. Periodic Acid Schiff (PAS) reaction is then used to stain basement membranes, glycogen and neutral mucosubstances pink to red. Mixtures of neutral and acidic mucosubstances will appear purple due to positive reactions with both Alcian Blue and PAS.

Used to stain myelin and phospholipids.

Three-step procedure for differentiation of morphological cell types in peripheral blood smears. Staining characteristics are similar to the traditional Wright’s and Wright-Giemsa stains.

Polysciences, Inc. Lithium Blue is a gentle yet rapid formulation for bluing hematoxylins in only 5 minutes. Lithium Blue is buffered at an alkalinity of (pH = 8.0)

Chromic acid oxidation forms aldehydes from fungal cell wall polysaccharide components, which are subsequently demonstrated by reduction of an alkaline hexamine-silver complex. The reaction may be compared to that of the periodic acid Schiff reaction.

Intended for the use in the histological visualization of Argentaffin cells and Melanin in paraffin or frozen sections.

Useful for staining fresh, fixed, unembedded or plastic-embedded sections of bone. Villanueva Osteochrome Bone Stain gives uniform and reproducible results. Useful in studying biopsy or postmortem tissue.

This stain is useful in demonstrating atrophy of elastic tissue in cases of emphysema, thinning and loss of elastic fibers in arteriosclerosis and other vascular diseases. With increasing age, changes such as splitting and fragmentation occur, these changes are most obvious in the skin which becomes wrinkled and rather loose-fitting.

PAS techniques are used to demonstrate polysaccharides, neutral mucosubstances and basement membranes primarily in tissue. The PAS reagent is also called Fuelgen Stain for the demonstration of DNA with a different protocol. Kidney is the most sensitive control. The demonstration of glycogen is best represented by a section of liver with a digestion step used as a negative control in the staining.

Prussian Blue or Perls’ reaction is used to demonstrate ferric iron and ferritin. This is not a true staining technique rather, it is a histochemical reaction.

The protein is split off by the hydrochloric acid, allowing the potassium ferrocyanide to combine with the ferric iron. This forms the ferric ferrocyanide or Prussian Blue.

For staining bacteria from cultures or specimens by the differential Gram stain method. Kit performs 150 tests.

Complete kit to aid in the staining of tissue for Amyloidosis. Amyloid Stain Kit used in the detection of amyloid FFPE as well as frozen tissue sections cut at 10 microns. The amyloid stains red and the nuclei stains blue. Control tissue is Alzheimer’s or other known amyloidosis.

A nuclear staining solution containing, hematoxylin, ferric chloride and hydrochloric acid used in many non-routine techniques (special stains) because, it resists decolorization in acidic staining solutions. Ferric Chloride is a strong oxidizer, so it serves both as a mordant and oxidizer for Weigert's Hematoxylin.

Acid Fuchsin is one of the dyes used in the Masson's Trichrome Staining technique. This method is commonly used to stain tissue sections in the Histology and Cytology Laboratory in order to distinguish muscle from collagen. The muscle stains red with the acid fuchsin and the collagen is stained green or blue with light green SF yellowish or methyl blue.

Used in combination with methyl green for the selective and differential staining of nucleic acids. The pyronin Y stains RNA red, while the methyl green stains DNA green. The combined methyl green-pyronin Y stain is a useful histochemical reagent. Pyronin Y can also be used as a tracking dye for polyacrylamide gel electrophoresis.

λmax 552nm

DNA intercalating dye. A grade of acridine orange of exceptionally high purity, suitable for quantitative work. Free of inorganic salts. A specific stain for RNA, used as a 2% solution containing 1% lanthanum acetate in 15% acetic acid.

Useful for staining blood films and malarial parasites in blood films.

Polyene antibiotic fluorochrome for cholesterol determination. Used as an antifungal agent.

A post-electrophoresis stain for lipoproteins on cellulose acetate plates.

(Solvent red 27; Sudan red 5B; Ceres red 5B)

Cardiac muscle stain.

(Direct red 80)

(Food green 3). Sensitive stain for proteins in polyacrylamide gels. Especially suitable in isoelectric focusing. Also suitable for use as a cytological counterstain, and mammalian tissue stain for muscle and collagen.

λ max 622-626nm.

Protein stain for SDS gels. Used in dye binding techniques for protein quantification.

(Coomassie blue® G250; Brilliant blue G250; Acid blue 90)

λmax 610nm

Rapid acting and sensitive dye for SDS gels.

(Coomassie blue® R250; Brilliant blue R250; Acid blue 83)

λmax 588nm

Nuclear protein stain and glycogen stain. Also a general tissue stain for human, animal and VIR histology.

For use as a buffer in May-Grünwald, Wright Stain, Wright-Giemsa, Giemsa and Leishman staining procedures.

This Phosphate Buffer yields satisfactory staining results every time when used with the Wright staining method.

Wright Stain is a dual purpose stain used for staining blood smears and bone marrow aspirates. Ready-to-use solution makes the differentiation of human blood cells much easier to identify.

This Phosphate Buffer yields satisfactory staining results every time when used with Wright-Giemsa stains.

Contains no acetic acid. Used as counterstain with hematoxylin. Ready to use with any automated stainer.

Used as counterstain with hematoxylin; contains acetic acid. Does not contain isopropanol or methanol. Ready to use with any automated stainer.

Used in Tzanck preparations of herpetic lesions and to differentiate acidophilic and basophilic structures. Stain can be directly applied to frozen sections, epoxy or JB-4® embedded sections and utilized as a general cytoplasmic and nuclear stain.

OG-6 is a cytoplasmic counterstain solution used in sequence with EA (EA 50, EA 65 or EA36) in the Papanicolaou staining method for clinical cytology. Gill’s modified OG-6 is stable in solution and gives predictable high quality staining results not previously possible with other formulations.

Orange-6 and EA are the two cytoplasmic counterstain solutions that are used sequentially in the Papanicolaou staining method for clinical cytology. Gill’s modified OG-6 and EA are stable in solution and give predictable high quality staining results not previously possible with other formulations.

Polysciences, Inc. Gold Standard Series Hematoxylin and Eosin products offer you the highest quality for routine staining. Strict quality control for all Gold Standard Series Routine Stains ensures lot-to-lot consistency. Stains are packaged in convenient plastic recyclable containers.

Gold Standard Gill's #3 is the formulation of choice for routine histological staining when a darker nuclear stain is desired.

Gold Standard Gill's #2 should be used when a stronger or darker nuclear stain is required for cytology samples or for immunohistochemical counterstaining.

Polysciences, Inc. Gold Standard Series Hematoxylin and Eosin products offer you the highest quality for routine staining. Strict quality control for all Gold Standard Series Routine Stains ensures lot-to-lot consistency. Stains are packaged in convenient plastic recyclable containers.

Giemsa stain is a classical blood film stain for peripheral blood smears and bone marrow specimens, used to visualize chromosomes, stains fungus histoplasma and identifies mast cells.

To be used in conjunction with our May-Grünwald Stain Solution, Cat. #24981

Wright-Giemsa Stain Solution is a dual purpose stain useful for blood films, parasites and bone marrow aspirates. Prepared from certified dyes.

Ready-to-use solution makes the differentiation of human blood cells much easier to identify.

For use in both cytology and histology. General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining. Used as a counterstain for immunohistochemistry procedures.

A gentle formulation of "bluing" reagent for those specimens that may be affected by more harsh "bluing" agents. Our premixed and ready to use Scott's Bluing Reagent provides rapid bluing and crisp nuclear detail obtained with routine Hematoxylin and Eosin stains. For use in both Cytology and Histology.

Periodic Acid Schiff Methenamine Silver Stain for Basement Membranes

A new and improved Jones PAS-M technique that utilizes an enhancer to accelerate the methenamine silver reaction with the glomerular basement membranes, while reducing the time to achieve results better than the original Jones PAS-M and a more stabilized silver solution. Used in kidney biopsies, Jones PAS-M may also be used for plastic sections for glomerular and tubular basement membranes.

Since plastic sections have a tendency to come off slides in staining procedures that require heat we recommend using Tissue Tack Microscope Slides Cat. #24216

Identifies reticulin fibers in tissue sections liver, kidney and spleen. Reticulin is a type III collagen found in the basement membrane of many organs and provides structural integrity. 

Used as a substrate for the histochemical demonstration of γ-glutamyl transpeptidase activity.

Used for the detection of collagen fibers in tissues such as skin, heart, etc. on formalin-fixed, paraffin-embedded sections and may be used for frozen sections as well. Collagen fibers stained blue, nuclei stained black and cytoplasm, muscle, erythrocytes stained red.

Available in 100ml and 500ml

May-Grünwald Stain Solution is a classic hematology stain that produces dense coloration in the staining of peripheral blood smears and bone marrows.

Used to stain DNA, RNA, and protein. Also useful for staining acid polysaccharides.

DNA stain blue

RNA stain bluish-purple

Protein stain red

The Bielschowsky Stain is a very useful method in detecting the twist and clumping of neurons in the brain, otherwise known as, neurofibrillary tangles and senile plaques, which are signs of the Alzheimer’s disease. The Bielschowsky stain, along with other diagnostic test, can assist scientist and researchers in the mission for finding a cure for this disease of memory loss.

Electron dense metal stain.

(12-Molybdophosphoric acid)

Oil Red O is excellent for the demonstration of general localization of fats in frozen tissue sections. 

Reduced ethidium bromide. A vital stain. Enters and stains living cells without cellular trauma.

Stable DNA stain.

Dye for hemodynamic studies. Invest.

Cardiogreen; Fox green λ max 775nm

Saffron is used in a variety of histological staining methods including the Hematoxylin, Phloxine and Saffron (HPS) staining technique.

A STAT staining method for unfixed and fixed tissue. Stains nuclei more strongly than cell cytoplasm.Excellent dye for nuclear and nucleolar details. Can be used to demonstrate erythrocyte alterations/inclusions and some erythrocyte parasites, as well as to visualize reticulocytes.

Electron microscopy stain.

Fluorescent dye for reticulocyte analysis. Also useful for Plasmodium species analysis.

λ max: 512nm

The Fungi-Fluor^® Kit for Fungal Detection offers a quick fluorescent stain/counterstain procedure for various fungal organisms. The kit can be used to screen a variety of specimen types, such as sputum and skin scrapings, for fungal detection.

Pneumocystis jiroveci (previously named P. carinii) is an organism that can cause Pneumocystis carinii pneumonia (PCP) in immunocompromised patients. PCP, long associated with morbidity and mortality with HIV-infected (AIDS) patients, is now increasing in prevalence among the non-HIV infected immunosuppressed population. Early detection allows the introduction of appropriate treatment and may improve chances of patient survival. The Fungi Fluor^® Pneumocystis kit offers a fast, fluorescent staining procedure for Pneumocystis carinii in bronchial specimens.

Product is for research use only in Europe.

Gomori’s One Step Trichrome refers to the multiple stain reaction of this reagent only. However, adequate staining of all cell components requires several other solutions to obtain balance in the tissue. The mordant with Bouin’s Fixative is used to drop the pH and for protein interaction in the section. While this mechanism is not clear the stain works best with this step included.

Silver staining is a highly sensitive method for detecting proteins in polyacrylamide slab gels. Most silver staining protocols are time consuming, complicated, and dependent upon the purity of the reagents. Polysciences’ Silver Stain kit is simple, stable, controllable, and very rapid. Our method is sensitive to proteins in the nanogram range and may be used either before or after Coomassie blue staining. Staining for proteins is initiated in an alkali environment.

Von Kossa Method for Calcium Kit demonstrates only calcium phosphate and calcium carbonate salts. Note that when used in skin specimens that the silver will reduce the melanin in the skin, to also give a black deposit.

(9-Amino-5-imino-5H-benzo[a]phenoxazine acetate salt) Cresyl Violet Acetate is a certified solid dye used for the preparation of a staining solution to demonstrate the presence of Nissl substances in neurons and cell nuclei.

Equal to or better than ACS specifications. Silver stain techniques are widely used to detect nanogram quantities of proteins following electrophoresis. Silver nitrate is the silver source in most silver stain procedures.

A metachromatic, cationic thiazine dye that is widely used in in vitro biological applications. It has also been used in techniques for DNAase detection.

λmax 626nm

Blood stain similar to Wright’s stain. Also useful for staining bone sections.

(Taylor’s blue; 3,7’-Bis(dimethylamino)-1,9-dimethyldiphenothiazin-5-ium chloride) λ max 649nm

Used in dye binding assays for glycosaminoglycans.

Collagen Type I and III Staining

Our Picrosirius Red Stain binds specifically to collagen fibrils of varying diameter that is used to distinguish collagen Type I from Type III. Picrosirius Red Stain will quantify the amount of collagen in a given area of myocardial tissue. (i.e. the collagen area fraction)

Cytoplasmic counterstain. Component of Wright stain and Tetrachrome stain.

(Eosin yellowish; Acid red 87; 2’,4’,5’,7’-Tetrabromo-fluorescein, disodium salt)

λmax: 517nm

Uvitex 2B fluorescent dye will bind to the chitin in fungal walls and is highly selective for fungi and algae in tissue sections. It has a slower fade rate than more commonly used fungal fluorescent dyes.