DOTAP Chloride

CAS Number:
Linear Formula:


Grouped product items
Catalog Number Unit Size Price QTY
14475-25 25mg
14475-100 100mg
14475-500 500mg
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DOTAP Chloride (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride) is a widely used cationic liposome-forming compound for in vitro and in vivo transfection of DNA, RNA and other negatively charged molecules. DOTAP encapsulates the nucleic acids to form a stable complex spontaneously by means of electrostatic interaction, facilitates cell attachment, internalization by endocytosis as well as endosomal escape by proton sponge. It has been used in the formation of Lipid Nanoparticles (LNPs) or Nanostructured Lipid carriers (NLCs) in the development of mRNA vaccines such as COVID-19 vaccine and drug delivery systems. This product is for research use only.

Contact us at [email protected] to know about our cGMP grade DOTAP Chloride manufactured under 21 CFR part 210,211.

Key features:

  • High efficiency and reproducibility
  • Cost effective and easy to use
  • Scalable
Transfection of DNA, RNA, and oligonucleotides, Lipid Nanoparticles (LNPs), Nanostructured Lipid Carriers (NLCs), mRNA vaccine development, Drug delivery system development
Shipping Requirements:
Dry Ice
White to off white solid
Molecular Weight:

Jain S, Kumar S, Agrawal AK, Thanki K, Banerjee UC. Enhanced transfection efficiency and reduced cytotoxicity of novel lipid-polymer hybrid nanoplexes. Mol Pharm. 2013;10(6):2416-2425. doi:10.1021/mp400036w

Simberg, Dmitri, et al. "DOTAP (and other cationic lipids): chemistry, biophysics, and transfection." Critical Reviews™ in Therapeutic Drug Carrier Systems 21.4 (2004).

Store powdered material at -20°C, protect from moisture and light.
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