DOTAP Chloride (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride) is a widely used cationic liposome-forming compound for in vitro and in vivo transfection of DNA, RNA and other negatively charged molecules. DOTAP encapsulates the nucleic acids to form a stable complex spontaneously by means of electrostatic interaction, facilitates cell attachment, internalization by endocytosis as well as endosomal escape by proton sponge. It has been used in the formation of Lipid Nanoparticles (LNPs) or Nanostructured Lipid carriers (NLCs) in the development of mRNA vaccines such as COVID-19 vaccine and drug delivery systems. This product is for research use only.
Contact us at [email protected] to know about our cGMP grade DOTAP Chloride manufactured under 21 CFR part 210,211.
- High efficiency and reproducibility
- Cost effective and easy to use
Jain S, Kumar S, Agrawal AK, Thanki K, Banerjee UC. Enhanced transfection efficiency and reduced cytotoxicity of novel lipid-polymer hybrid nanoplexes. Mol Pharm. 2013;10(6):2416-2425. doi:10.1021/mp400036w
Simberg, Dmitri, et al. "DOTAP (and other cationic lipids): chemistry, biophysics, and transfection." Critical Reviews™ in Therapeutic Drug Carrier Systems 21.4 (2004).