Polyethylenimine, Linear, MW 25000, Transfection Grade (PEI 25K™)
PEI 25K is a powerful, trusted, and cost-effective transient transfection reagent. In HEK293 and CHO expression systems, PEI offers consistently high gene expression on a wide scale (96 well plates up to 100 L bioreactors). Each year, more researchers and companies turn to Polysciences PEI to gain a critical edge in their work. Relative to most other options, using PEI to prepare transfection reagents in-house can offer as much as a 40% reduction in total transfection costs.
Insoluble In: benzene, ethyl ether and acetone
Bang, S., Li, J., Zhang, M., Cui, R., Wu, X., Xin, Z., ... & Zhang, H. (2020). The Clinical Relevance and Function of Krüppel-Like Factor 16 in Breast Cancer. Cancer Management and Research, 12, 6373.
Baranyi, L., Doering, C. B., Denning, G., Gautney, R. E., Harris, K. T., Spencer, H. T., ... & Dropulic, B. (2013). Rapid generation of stable cell lines expressing high levels of erythropoietin, factor VIII, and an antihuman CD20 antibody using lentiviral vectors. Human Gene Therapy Methods, 24(4), 214-227.
Durocher, Y., Perret, S., & Kamen, A. (2002). High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic acids research, 30(2), e9-e9.
Li, J., Yu, W., Ge, J., Zhang, J., Wang, Y., Wang, P., & Shi, G. (2020). Targeting eIF3f Suppresses the Growth of Prostate Cancer Cells by Inhibiting Akt Signaling. OncoTargets and therapy, 13, 3739.
Sanzhez-Mata, A., Ferez-Gomez, A., & Gonzalez-Muñoz, E. (2020). Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs. STAR protocols, 1(3), 100183.
Schmitz, C., Pepelanova, I., Seliktar, D., Potekhina, E., Belousov, V. V., Scheper, T., & Lavrentieva, A. (2020). Live reporting for hypoxia: Hypoxia sensor–modified mesenchymal stem cells as in vitro reporters. Biotechnology and Bioengineering, 117(11), 3265-3276.
Siegman, S., Truong, N. F., & Segura, T. (2015). Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta biomaterialia, 28, 45-54.
Thomas, M., Lu, J. J., Ge, Q., Zhang, C., Chen, J., & Klibanov, A. M. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proceedings of the National Academy of Sciences, 102(16), 5679-5684.
Trefny, M. P., Kaiser, M., Stanczak, M. A., Herzig, P., Savic, S., Wiese, M., ... & Zippelius, A. (2020). PD-1+ natural killer cells in human non-small cell lung cancer can be activated by PD-1/PD-L1 blockade. Cancer Immunology, Immunotherapy: CII.
Wulhfard, S., Baldi, L., Hacker, D. L., & Wurm, F. (2010). Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of biotechnology, 148(2-3), 128-132.