Distearoylphosphatidylcholine (DSPC)
C44H88NO8P
Distearoylphosphatidylcholine (DSPC) is a zwitterionic phospholipid that is used in the preparation of liposomes for transfection and drug delivery applications. DSPC functions to enhance encapsulation efficiency and liposome stability which enables greater effectiveness in bioprocessing. Lipopolyplexes containing DSPC have been found to have less cytotoxicity and therefore greater cell viability when compared to other phospholipid-based systems. DSPC has been receiving increasing attention as a valuable component in forming lipid nanoparticles (LNPs) in the growing field of mRNA vaccines. LNPs facilitate the safe and effective delivery of mRNA to target cells, thus enabling therapeutic action. As of 2021, both Moderna and Pfizer-BioNTech COVID-19 vaccines utilize DSPC as a critical part of their lipid formulations.
Key Benefits:
- Increases liposome rigidity and stability
- Less cytotoxicity
- High transfection efficiency
Synonyms: DSPC, (2R)-2,3-Bis(octadecanoyloxy)propyl 2-(trimethylazaniumyl)ethyl phosphate, 1,2-Distearoyl-sn-glycero-3-phosphocholine
Contact us at [email protected] to know about our cGMP grade DSPC manufactured under 21 CFR part 210,211.
Anderson M, Omri A. The effect of different lipid components on the in vitro stability and release kinetics of liposome formulations. Drug Deliv. 2004 Jan-Feb;11(1):33-9. doi: 10.1080/10717540490265243. PMID: 15168789.
Fraga M, Bruxel F, Lagranha VL, Teixeira HF, Matte U. Influence of phospholipid composition on cationic emulsions/DNA complexes: physicochemical properties, cytotoxicity, and transfection on Hep G2 cells. Int J Nanomedicine. 2011;6:2213-2220. doi:10.2147/IJN.S22335
Hashemzadeh, H., Javadi, H. & Darvishi, M.H. Study of Structural stability and formation mechanisms in DSPC and DPSM liposomes: A coarse-grained molecular dynamics simulation. Sci Rep 10, 1837 (2020). https://doi.org/10.1038/s41598-020-58730-z
Kim, J., Jozic, A. & Sahay, G. Naturally Derived Membrane Lipids Impact Nanoparticle-Based Messenger RNA Delivery. Cel. Mol. Bioeng. 13, 463 -474 (2020). https://doi.org/10.1007/s12195-020-00619-y
