Many opportunistic fungal infections develop in immunocompromised HIV-infected (AIDS) patients and also increasingly among the non-HIV infected immunosuppressed population. A number of these are caused by various fungal species including Candida, Aspergillus, Histoplasma, and Coccidioides.
The Fungi-Fluor® Kit for Fungal Detection offers a quick fluorescent stain/counterstain procedure for various fungal organisms. The kit can be used to screen a variety of specimen types, such as sputum and skin scrapings, for fungal detection.
Fungi-Fluor® Staining Solution is a 0.05% solution of high purity Cellufluor, an active ingredient of Calcofluor white fluorescent stain, in deionized water with potassium hydroxide added as a clearing agent. Fungi-Fluor® Counterstaining Solution B is an aqueous solution of Evans Blue dye, which is used to reduce background fluorescence. The Fungi-Fluor® Kit can be used for the rapid identification of various fungal infections in fresh or frozen clinical specimens, paraffin or glycol methacrylate embedded tissues. Typical specimens include sputum, bronchoalveolar lavage (BAL), bronchial wash and tissue biopsies.Advantages:
- More accurate diagnosis than KOH preps
- Rapidly offers greater morphologic detail than PAS or silver stains
- Counterstain greatly reduces background fluorescence
- Simplicity and rapidity over immunostains
- One kit stains over 500 slides
- Packaged in convenient dropper bottles
- Fresh or frozen clinical specimen
- Paraffin or GMA-embedded tissues
Harrington BJ, Hageage GJ Jr., (2003) Calcofluor White: A Review of its Uses and Applications in Clinical Mycology and Parasitology. Lab Medicine 34(5):361-367.
Alvarez F, Bandi V, Stager C, Guntupalli KK. (1997) Detection of Pneumocystis carinii in tracheal aspirates of intubated patients using calcofluor-white (Fungi-Fluor) and immunofluorescence antibody (Genetic Systems) stains. Critical Care Medicine 25(6):948-952
Baselski VS, Robison MK, Pifer LW, Woods DR. (1990) Rapid detection of Pneumocystis carinii in bronchoalveolar lavage samples by using Cellufluor staining. J Clin Microbiol 28(2):393-394.
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- Exercise normal care
- Protect from light. Store at room temperature. Do not permit to freeze